Integrative modeling of Transcription Factor cooperativity and its effects on phenotypic variability

The regulation of biological processes relies on a complex nucleotide code embedded in our DNA. Its decoding and interpretation is the main task of Transcription Factors (TFs), which altogether enable the recognition and modulation of gene expression. Whenever factors bind to DNA, a set of additional criteria and conditions need to be satisfied, such as TF concentration, DNA openness, and cooperativity with other binding factors. Such combinations of DNA-bound TFs, as well as their structural and functional cooperativity, allow a more fine-grained control of gene expression due to subtle changes in specificity in both DNA recognition and functional outcomes. This thesis explores the prediction of structural TF cooperativity and its biological consequences. Additionally, examples of functional cooperativity are presented and discussed in the context of neuronal activity and reprogramming. Altogether, this dissertation provides an extensive set of insights to better understand the complex interplay between TFs cooperativity and phenotypes

El Fuero Viejo de Castilla : sacado, y comprobado con el exemplar de la misma obra, que existe en la Real Biblioteca de esta Corte, y con otros MSS.

Copia digital. Valladolid : Junta de Castilla y León. Consejería de Cultura y Turismo, 2009-201

Cortes celebradas en los reynados de D. Sancho IV y de D. Fernando IV

Copia digital. Valladolid : Junta de Castilla y León. Consejería de Cultura y Turismo, 2009-2010Marca de imp. en port.Viñetas al final de cada capítul

El Ordenamiento de leyes que D. Alfonso XI hizo en las Cortes de Alcala de Henares, el año de mil trescientos y quarenta y ocho

Copia digital. Valladolid : Junta de Castilla y León. Consejería de Cultura y Turismo, 2009-2010Marca de imp. en port.Viñetas al final de cada capítul

4to. Congreso Internacional de Ciencia, Tecnología e Innovación para la Sociedad. Memoria académica

Este volumen acoge la memoria académica de la Cuarta edición del Congreso Internacional de Ciencia, Tecnología e Innovación para la Sociedad, CITIS 2017, desarrollado entre el 29 de noviembre y el 1 de diciembre de 2017 y organizado por la Universidad Politécnica Salesiana (UPS) en su sede de Guayaquil. El Congreso ofreció un espacio para la presentación, difusión e intercambio de importantes investigaciones nacionales e internacionales ante la comunidad universitaria que se dio cita en el encuentro. El uso de herramientas tecnológicas para la gestión de los trabajos de investigación como la plataforma Open Conference Systems y la web de presentación del Congreso http://citis.blog.ups.edu.ec/, hicieron de CITIS 2017 un verdadero referente entre los congresos que se desarrollaron en el país. La preocupación de nuestra Universidad, de presentar espacios que ayuden a generar nuevos y mejores cambios en la dimensión humana y social de nuestro entorno, hace que se persiga en cada edición del evento la presentación de trabajos con calidad creciente en cuanto a su producción científica. Quienes estuvimos al frente de la organización, dejamos plasmado en estas memorias académicas el intenso y prolífico trabajo de los días de realización del Congreso Internacional de Ciencia, Tecnología e Innovación para la Sociedad al alcance de todos y todas

Cartas Eruditas de algunos literatos españoles

Melchor de Azagra es seudónimo de Ignacio de Jordán de Asso y del RíoSign.: [calderón]\p6\s, A-I\p8\s, K\p2\sAnte

Structural characterization of lignin in different eucalypt species

Rencoret, Jorge et al.-- 4 páginas, 1 figura, 2 tablas, 12 referencias.-- Congreso celebrado del 26-29 de agosto 2008, en Stockholm, Sweden.The chemical structure of milled-wood lignins from Eucalyptus globulus, E. nitens, E. maidenii, E. grandis and E. dunnii was investigated. The lignins were characterized by analytical pyrolysis, thioacidolysis and 2D-NMR that confirmed the predominance of syringyl over guaiacyl units, and only showed traces of p-hydroxyphenyl units. The HSQC-NMR spectra showed a predominance of β-O-4' ether linkages (66-72% of total side-chains), followed by β-β' resinol-type linkages (16-19% of side-chains) and lower amounts of β-5' phenylcoumaran-type (3-7% of side-chains) and β-1' spirodienone-type linkages (1-4% of side-chains). The analysis of desulphurated thioacidolysis dimers provided additional information on the carbon-carbon and diaryl ether bonds forming the most condensed moiety of the eucalypt lignins, and the type of units involved in each of the above linkage types. Interestingly, 93-94% of the total β-β' dimers included two syringyl units indicating that most of the β-β' substructures identified in the HSQC spectra were of the syringaresinol type. Moreover, three isomers of a major trimeric compound tentatively identified as arising from a β-β' syringaresinol substructure attached to a guaiacyl unit through a 4-O-5' linkage were found.This study has been funded by the Spanish project AGL2005-01748, the CSIC project 2006-4-0I-039 and the BIORENEW EU-Project (NMP2-CT-2006-026456). J.R. thanks the CSIC for a I3P fellowship and the COST Action E-41 for funding an stay at KTH.Peer reviewe

Occurrence and structural characteristics of highly acylated (acetylated and/or p-coumaroylated) native lignins from diverse herbaceous plants

We have studied the occurrence of native acylated (with acetates and/or p-coumarates) lignin in a large set of vascular plants, including both angiosperms and gymnosperms by DFRC and HSQC-NMR. Acylated lignin units were found in all angiosperms, including mono- and eudicotyledons, but were absent in the gymnosperms analyzed. Acylation occurred exclusively at the γ-carbon of the lignin-side chain and predominantly on syringyl units. In some plants (e.g. sisal, kenaf, abaca, curaua or hornbeam), lignin acylation occurred at a very high extent, up to 80 %. The structure of these highly-acylated lignins was characterized by a very high syringyl/guaiacyl ratio, a predominance of β-O-4´ linkages (up to 94% of all linkages) and a very low proportion of β-β´ linkages, which indeed are completely absent in the lignins from abaca and curaua. In all cases, acylation appears to occur at the monomer stage and sinapyl and coniferyl acetates have been demonstrated to behave as real lignin monomers participating in lignification.This study has been supported by the Spanish MEC (project AGL2005-01748), the EU contract NMP2-CT-2006-26456 (BIORENEW) and the CSIC project 2006-4-OI-39. JR thanks the Spanish CSIC for an I3P fellowship; GM thanks the Spanish Ministry of Education for a FPI fellowship.Peer reviewe

Fuero Viejo de Castilla

Sign.: [ ]\p2\s, a-g\p4\s, A-V\p4\s.Port. con grab. calc. : "Cruz inv. et sculp.

Fuero Viejo de Castilla

Sign.: [ ]\p2\s, a-g\p4\s, A-V\p4\s.Port. con grab. calc. : "Cruz inv. et sculp.